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alpha-D-Glucose-1-Phosphate (G1P)

Glucose-1-phosphate can be used as a substrate in the assay of the activities of phosphoglucomutase and UDP-glucose pyrophosphorylase and also in the preparation of other sugar phosphates.

1.Source: Disodium, tetrahydrate
   Form: Crystalline powder 
   Molecular Weight: 376.1 
   Solubility: Distilled water or dilute buffer 
   Stability: Store at 4 C (39 F) 
   Protein: 99-100% glucose-1-phosphate (assayed enzymatically) 
   Catalog No.: 101J0000 

Glucose-1-P is converted to Glucose-6-P with phosphoglucomutase (EC Glucose-6-P is oxidized by NADP+ and glucose-6-P dehydrogenase (EC to 6-phosphogluconate (Methods of Enzymatic Analysis, Bergmeyer, H.U. ed. VOL 3, 1233, 1974, Academic Press, New York).

  1. 0.1 M Triethanolamine buffer containing 2 mM EDTA and 5 mM MgCl2, pH 7.6.
  2. 0.02 M NADP+ (17.4 mg/ml) in distilled water.
  3. Phosphoglucomutase (PGM). Prepare in buffer to yield a final concentration of 100 U/ml. Prepare fresh prior to assay.
  4. Glucose-6-P-dehydrogenase (G6PDH). Prepare in buffer to yield a final concentration of 100 U/ml. Prepare fresh prior to assay.
  5. 0.012 M Glucose-1-P (substrate). Dissolve 44.6 mg glucose-1-P, dipotassium dihydrate in 10 ml distilled water. Prepare fresh.

  1. Set spectrophotometer (equipped with a strip chart recorder and temperature control) at 37C and 340 nm.
  2. Into a cuvette, pipette the following in the amounts indicated:
    Triethanolamine buffer with EDTA and MgCl2 2.70 ml
    NADP+ 0.10 ml
    NADH 0.10 ml
    Glucose-6-P-DH 0.10 ml
    Glucose-1-P (substrate) 0.01 ml
  3. Incubate cuvette in the spectrophotometer at 37C for 5 minutes.
  4. Record the initial absorbance at 340 nm (Ai).
  5. Initiate the reaction by adding 0.10 ml of phosphoglucomutase solution to the cuvette.
  6. Follow the reaction for 10 minutes. Record the final absorbance at 340 nm (Af).


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