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beta-Nicotimamide Adenine Dinucleotide (NAD)
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NAD is used in the determination of amylase, creatine kinase,
transaminases, lactate dehydrogenase, phosphohexose isomerase,
ethanol, galactose, glucose, uric acid, L-lactate,
triglycerides and other enzymes and metabolites.
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1.Source: |
Monolithium salt
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Form: |
Crystalline powder
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Molecular Weight: |
663.4
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Solubility: |
Distilled water or dilute buffer
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Stability: |
Store at -20° C (-4° F)
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Purity (approx.): |
>95%
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Catalog No.: |
216J0000
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- Sodiumdiphophate/semicarbazide/substrate, pH 8.7: 3.33 g
Na4P2O7&10 H2O, 0.84 g semicarbazide&HCl, 0.17 glycine, 1.00 ml
ethanol with 80 ml distilled water. Adjust pH to 8.7 with 2 M NaOH;
adjust volume to 100 ml.
- Alcohol dehydrogenase, from yeast (30 mg protein/ml): 300 U/ml.
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- Dissolve 25 mg NAD in 25 ml distilled water in a volumetric flask.
- Set spectrophotometer (equipped with strip chart recorder and
temperature control) at 340 nm and 25°C.
- Into a cuvette, pipette the following:
Buffer (1) 3.00 ml
sample 0.10 ml
Mix and read the absorbance A1
- Start the reaction by adding 0.01 ml of Alcohol dehydrogenase
(2). Mix and read absorbance A2.
- Add another 0.01 ml Alcohol dehydrogenase. Mix and read
absorbance A3.
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