(L-Lactate: NAD+ oxidoreductase EC 1.1.1.27)
Lactate dehydrogenase (LDH) catalyzes the following reaction:
LDH
Pyruvate + NADH ------> Lactate + NAD+ + H+
LDH is a metabolic enzyme which is widely distributed in
nature. Mammalian LDH exists as five tetrameric isozymes
composed of combinations of two different subunits. The
isozymes differ in physical, immunological and catalytic
properties. LDH has a molecular weight of about 140,000.
LDH is of clinical significance in that the serum level of
certain isozymes suggests pathological changes in particular
tissues. It is also used extensively for the determination of
lactate and in coupled systems for detection of biological
metabolites.
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